产品说明
一般描述
Monoclonal antibody to fluorescein.
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.
Contents
Lyophilizate, stabilized
特异性
The monoclonal antibody reacts with free and bound fluorescein.
应用
Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
制备说明
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
- ELISA: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.
重悬
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Reconstitute for 15 minutes.
其他说明
仅用于生命科学研究。不可用于诊断。
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | B13-DE1, monoclonal |
| 形式 | lyophilized |
| 包装 | pkg of 100 μg |
| manufacturer/tradename | Roche |
| 同位素/亚型 | IgG1 |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H317 - H412 |
| 预防措施声明 | P261 - P273 - P280 - P333 + P313 - P362 + P364 - P501 |
| 危险分类 | Aquatic Chronic 3 - Skin Sens. 1 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 2 |
| 闪点(F) | does not flash |
| 闪点(C) | does not flash |
