Anti-Capsaicin Receptor Antibody, NT

产品说明

特异性

Capsaicin Receptor (VR1), N-terminal.

SPECIES REACTIVITIES:

Rat, mouse and primate. Other species have not been tested.

免疫原

Epitope: N-terminus
Synthetic peptide corresponding to the N-terminus of rat VR1.

应用

Immunoblotting: 1:1,000

Immunohistochemistry: 1:1,000

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5889

IMMUNOHISTOCHEMISTRY

Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5889. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

IMMUNOHISTOCHEMISTRY

VR1 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.

WESTERN BLOT

For details see Guo, A., et al., Eur J Neurosci (1999) 11:946-958.
This Anti-Capsaicin Receptor Antibody, N-terminus is validated for use in WB, IH for the detection of Capsaicin Receptor.
Research Category
Neuroscience
Research Sub Category
Neuroinflammation & Pain

Neurotransmitters & Receptors

外形

Liquid. Contains 0.05% sodium azide.

储存及稳定性

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息