Anti-DNA-PKcs Antibody, clone 3A9.1

产品说明

一般描述

DNA-dependent protein kinase catalytic subunit (EC 2.7.11.1; UniProt P78527; also known as DNA-PK catalytic subunit, DNA-PKcs, DNPK1, p460) is encoded by the PRKDC (also known as HYRC, HYRC1, IMD26, XRCC7) gene (Gene ID 5591) in human. DNA-PK is a heterotrimer of PRKDC and the Ku p70/YRCC6-p86/XRCC5 dimer. It acts as a molecular sensor for DNA damage and is involved in DNA non-homologous end joining required for double-strand break (DSB) repair and V(D)J recombination. Upon binding to DNA, it expresses its catalytic properties. DNA-PKcs falls into the phosphatidylinositol (PI) 3-kinase family, however, biochemical assays indicate that it phosphorylates proteins and has no detectable activity toward lipids. DNA-PKcs is displays high similarity to PI kinase family members involved in cell cycle control, DNA repair, and DNA damage responses. Deficiency of DNA-PKcs is linked to severe combined immunodeficiency characterized by reduced or absent T and B cells, recurrent candidiasis, and lower respiratory tract infections. Ref.: Hartley, KO., et al. (1995). 82, 849-856.

特异性

Clone 3A9.1 detects DNA-PK catalytic subunit (DNA-PKcs), but not the regulatory subunit (Ku). Target epitope is present in both spliced isoforms of human DNA-PKcs reported by UniProt (P78527).

免疫原

GST-tagged recombinant fragment from the region between FAT and KIP-binding domain of human DNA-PKcs

应用

Detect DNA-PK catalytic subunit (DNA-PKcs) using this mouse monoclonal Anti-DNA-PKcs Antibody, clone 3A9.1, Cat. No. MABC1236, validated for use in Western Blotting.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA-PKcs in 10 µg of K562 cell lysate.

目标描述

~470 kDa observed. 469.1/466.5 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified.
Format: Purified
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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