Anti-3-PGDH Antibody, clone TK-08 13A8

产品说明

一般描述

D-3-phosphoglycerate dehydrogenase (EC1.1.1.95; UniProt O43175; also known as 3-PGDH, Epididymis secretory protein Li 113) is encoded by the PHGDH (also known as 3PGDH, HEL-S-113, NLS, NLS1, PGAD, PGDH, PDG, PGD, PGDH3, PHGDHD, SERA) gene (Gene ID 26227) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. L-serine is an important precursor for macromolecule biosynthesis. 3-PGDH catalyzes the first and rate-limiting step of the serine synthesis pathway from 3-phosphoglycerate to phospho-hydroxypyruvate and is important during tumor development. The PHGDH gene is frequently amplified and the enzyme is overexpressed in 70% of the estrogen receptor-negative breast cancers, 39% of melanoma, and cervical cancers.

免疫原

Recombinant protein corresponding to human 3-PGDH.

应用

Detect PHGDH using this mouse Monoclonal Anti-3-PGDH Antibody, clone TK-08 13A8, Cat. No. MABS1145, validated for use in Western Blotting.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected 3-PGDH in 10 µg HEK293 and Raji cell lysates.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 3-PGDH in 10 µg of HeLa cell lysate.

目标描述

~57 kDa observed

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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