Anti-Calreticulin Mouse mAb (FMC75)

产品说明

一般描述

Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.
Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.

免疫原

a recombinant protein consisting of human calreticulin fused to maltose binding protein
Human

包装

50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Enzyme Immunoassay (0.1 µg/ml)

Immunoblotting (0.5 µg/ml, colorimetric; see application references)

Immunocytochemistry (see application references)

Immunohistochemistry (see application references)

Flow Cytometry (see application references)

警告

Toxicity: Standard Handling (A)

外形

In PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells

其他说明

Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息