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Anti-Neural Cell Adhesion Molecule Antibody

品牌
Sigma-Aldrich
货号
AB5032
规格纯度
Chemicon®, from rabbit
参考价格
6526.19 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.

特异性

Expected to cross-react with Chicken, Human, and rat.
Neural Cell Adhesion Molecule (NCAM). MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.

免疫原

Highly purified chicken NCAM

应用

Detect Neural Cell Adhesion Molecule using this Anti-Neural Cell Adhesion Molecule Antibody validated for use in ELISA, IF, IH, FUNC & WB.
Research Category
Neuroscience
Immunohistochemistry:
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.

Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.

Function blocking:
A previous lot of this antibody was used in Function blocking.

ELISA:
0.3 μg/mL of a previous lot was used.

Optimal working dilutions must be determined by end user.
Research Sub Category
Growth Cones & Axon Guidance

质量

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.

目标描述

MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa A breakdown product of 160 kDa may also be visible.

外形

ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS containing no preservatives.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
Neuraminidase-treated mouse brain membrane.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, rat, chicken, human
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登记号NM_000615.5
NM_001076682.2
NM_181351.3
UniProt登记号P13592
运输dry ice
Gene Informationhuman ... NCAM1(4684)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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