产品说明
一般描述
Tumor necrosis factor a (TNF-a) is a multifunctional cytokine, majorly produced by activated macrophages and is involved in the pathogenesis of many acute and chronic inflammatory condition.
Recombinant, Tumor Necrosis Factor-α, human (hTNF-α), is produced in Pichia pastoris and purified by standard chromatographic techniques.
Primary structure: One polypeptide chain (157 amino acids), identical to natural hTNF-a.
特异性
Human hTNF-α is effective on human and mouse cells.
Species specificity: human, mouse
应用
Tumor Necrosis Factor-α (hTNF-α)
- Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
- Has activating and growth stimulating activities on a variety of normal cells.
- Has antiviral activity on many cell types in vitro.
- Can cause tumor necrosis of certain tumors in vivo.
生化/生理作用
Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.
质量
Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
序列
Chain Length 157 AA
One polypeptide chain (157 amino acids), identical to natural hTNF-α.
单位定义
EC50 definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).
外形
Solution, filtered through 0.2 μm pore size membrane.
制备说明
Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 ℃
It is recommended to store the solution in aliquots at -15 to -25 ℃.
Note: Avoid repeated freezing and thawing.
其他说明
For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng
产品性质
| 质量水平 | 100 |
| 生物来源 | human |
| 重组 | expressed in yeast |
| 测定 | ≥95% (SDS-PAGE) |
| 形式 | solution |
| 效能 | ≤0.01 ng/mL EC50 |
| specific activity | >1*10^8 U/mg (>1 x 10⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.) |
| 分子量 | 17,000 Da |
| 包装 | pkg of 1,000,000 U (10 μg, 1ml) |
| manufacturer/tradename | Roche |
| 储存条件 | avoid repeated freeze/thaw cycles |
| 杂质 | <0.1 EU/μgtested (LAL test) |
| UniProt登记号 | Q9UBM5 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... TNF(7124) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | does not flash |
| 闪点(C) | does not flash |
