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Anti-PCNA (Ab-1) Mouse mAb (PC10)

品牌
Sigma-Aldrich
货号
NA03
规格纯度
liquid, clone PC10, Calbiochem®
参考价格
6526.19 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).

免疫原

Epitope: Within amino acids 112-121
recombinant PCNA

包装

200 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoblotting (2.5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (not recommended with unconjugated antibody, see comments)

Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)

警告

Toxicity: Standard Handling (A)

分析说明

Positive Control
HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue

其他说明

Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci.96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA86 3189.
Bravo, R., et al. 1987. Nature (London)326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol.105, 1549.
Miyachi, K., et al. 1987. J. Immunol.121, 2228.
Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆PC10, monoclonal
形式liquid
包含≤0.1% sodium azide as preservative
species reactivitymouse, rat, yeast, human, zebrafish
manufacturer/tradenameCalbiochem®
储存条件do not freeze
同位素/亚型IgG2a
运输wet ice
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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