Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)

产品说明

一般描述

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.
Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.

免疫原

Epitope: HHHHHH
a recombinant protein containing a His•Tag sequence

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (0.1-0.4 g/ml)
Immunoblotting (1-4 g/ml)
Immunofluorescence (1-4 g/ml)
Immunoprecipitation (4 g/ml)

警告

Toxicity: Standard Handling (A)

外形

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

分析说明

Positive Control
Proteins containing a His•Tag sequence

其他说明

Zentgraf, H., et al. 1995. Nucl. Acids. Res.23, 3347.
Gu, J., et al. 1994. BioTechniques17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol.68, 766.
Lu, T., et al. 1993. Anal. Biochem.213, 318.
Garner, J., et al. 1992. Cell69, 833.
Hochuli, E., et al. 1987. J. Chromatogr.411, 177.
Antibody should be titrated for optimal results in individual systems.

法律信息

Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息