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Anti-MBP (Citrulline R130) Antibody, clone 3C6

品牌
Sigma-Aldrich
货号
MABT1488
规格纯度
clone 3C6, from mouse
参考价格
1415.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Myelin basic protein (UniProt: P02686; also known as MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein) is encoded by the MBP gene (Gene ID: 4155) in human. MBP is a homodimeric protein that is found in both the central and the peripheral nervous system. It is one of the most abundant protein component of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane. It is responsible for adhesion of the cytosolic surfaces of multilayered compact myelin. MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. MBP expression begins abruptly in 14-16-week-old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. MBP citrullination by peptidylarginine deiminase (PAD) leads to incomplete protein-lipid bilayer interactions and increases its vulnerability to proteolytic enzymes, resulting in disorganization of the myelin sheath in the central nervous system. Citrullinated MBP (citMBP) has been suggested to be a hallmark of demyelination. MBP citrullination has frequently been demonstrated in various CNS disorders. citMBP at R25, R122, and R130 is reported in the brains of subjects with Creutzfeldt-Jakob disease (CJD). Clone 3C6 specifically recognizes citMBP at R130. (Ref.: Jang, B., et al. (2017). Mol. Neurobiol. 55(4); 3172-3184; Boggs, JM (2006). Cell. Mol. Life Sci. 63(17); 1945-1961).

特异性

Clone 3C6 is a mouse monoclonal antibody that detects human and murine myelin basic protein citrullinated on residue 130. It targets an epitope with in 19 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids from isoform 5 of human Myelin Basic Protein with citrullination on residue 130.

应用

Immunohistochemistry Analysis: A representative lot detected MBP (Citrulline R130) in Immunohistochemistry applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MBP (Citrulline R130) in Myelin basic protein (MBP) treated with human PAD2 (Courtesy of Byungki Jang, Ph.D./Eun-Kyoung Choi, Ph.D., Ilsong Institute of Life Science, Hallym University, South Korea).

Western Blotting Analysis: A representative lot detected MBP (Citrulline R130) in Western Blotting applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).

ELISA Analysis: A representative lot detected MBP (Citrulline R130) in ELISA applications (Jang, B., et. al. (2018). Mol Neurobiol. 55(4):3172-3184).
Research Category
Cell Structure
Anti-MBP (Citrulline R130), clone 3C6, Cat. No. MABT1488, is a mouse monoclonal antibody that detects Myelin basic protein citrullinated on residue 130 and has been tested for use in ELISA, Immunohistochemistry, and Western Blotting.

质量

Evaluated by Western Blotting in GST-tagged myelin basic protein (MBP) treated with human PADI-2.

Western Blotting Analysis: 4 µg/mL of this antibody detected MBP (Citrulline R130) in GST-tagged myelin basic protein (MBP) treated with human Peptidyl Arginine Deiminase 2 (PAD-2)(200 ng for 4 h at 37°C).

目标描述

~41 kDa observed; 18.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆3C6, monoclonal
species reactivityhuman, mouse
包装antibody small pack of 25 μg
technique(s)ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_001020252
UniProt登记号P02686

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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