Anti-Dopamine D₃ Receptor Rabbit pAb

产品说明

一般描述

Anti-Dopamine D₃ Receptor, rabbit polyclonal, recognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~50 kDa D₃ dopamine receptor protein.
Recognizes the dopamine D₃ receptor in rat brain. Does not cross-react with other dopamine receptors.

免疫原

a synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor

包装

100 μL in Plastic ampoule

应用

ELISA (see comments)

Flow Cytometry (1:100, see application references)

Immunoblotting (1:500)

Immunohistochemistry (1:5000)

警告

Toxicity: Standard Handling (A)

重悬

Reconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

其他说明

This antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息