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Anti-AnkyrinG Mouse mAb (463)

品牌
Sigma-Aldrich
货号
NB20
规格纯度
liquid, ≥95% (SDS-PAGE), clone 463
参考价格
6022.61 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of AnkyrinG.
Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.
Recognizes the ~190 kDa ankyrinG protein in brain extracts. Reacts with all spliced forms of ankyrinG.

  • Antibody Target Gene Symbol: ANK3
  • Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
  • Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
  • Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057)
  • Mu Entrez ID: 11735
  • Rat Entrez ID: 361833
  • 免疫原

    a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG
    Human

    包装

    100 μg in Plastic ampoule
    Please refer to vial label for lot-specific concentration.

    应用


    Frozen Sections (1-2 g/ml)
    Immunoblotting (2-5 g/ml)
    Immunofluorescence (1-2 g/ml)

    警告

    Toxicity: Standard Handling (A)

    外形

    In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

    分析说明

    Positive Control
    Mouse or rat brain extract or unmyelinated axons

    其他说明

    AnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
    Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement.
    Kunimoto, M., et al. 1995. FEBS Lett.357, 217.
    Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.
    Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.
    Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.
    Otto, E., et al. 1991. J. Cell Biol.114, 241.
    Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.

    法律信息

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    产品性质

    质量水平100
    生物来源mouse
    抗体形式purified antibody
    antibody product typeprimary antibodies
    克隆463, monoclonal
    测定≥95% (SDS-PAGE)
    形式liquid
    包含≤0.1% sodium azide as preservative
    species reactivitymouse, canine, rat, human
    manufacturer/tradenameCalbiochem®
    储存条件do not freeze
    同位素/亚型IgG1
    运输wet ice
    储存温度2-8℃

    安全信息

    储存分类代码12 - Non Combustible Liquids
    WGKnwg
    闪点(F)Not applicable
    闪点(C)Not applicable

    Sigma-Aldrich

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