产品说明
一般描述
Anti-BRCA1 (Ab-1), mouse monoclonal, clone MS110, recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. It is validated for WB, IF, IP, and frozen and paraffin sections.
Recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not cross-react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.
免疫原
Epitope: within the N-terminal 304 amino acids of BRCA1
Human
recombinant human BRCA1
包装
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Frozen Sections (see application reference)
Immunoblotting (2 µg/ml)
Immunofluorescence (see application reference)
Immunoprecipitation (see application reference)
Paraffin Sections (see application reference)
警告
Toxicity: Standard Handling (A)
外形
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
分析说明
Positive Control
MCF7 cells
其他说明
Scully, R., et al. 1996. Science272, 123.
Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.
Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.
Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet.11, 113.
Merajver, S.D., et al. 1995. Clin. Can. Res.1, 539.
Merajver, S.D., et al. 1995. Nat. Genet.9, 439.
Struewing, J.P., et al. 1995. Nat. Genet.11, 198.
Thompson, M.E., et al. 1995. Nat. Genet.9, 444.
Futreal, P.A., et al. 1994. Science266, 120.
Miki, Y., et al. 1994. Science266, 66.
Wilson, C.A., et al. 1999. Nat. Genet.21, 236.
Antibody should be titrated for optimal results in individual systems.
法律信息
Sold under license of U.S. Patents 5,753,441 and 6,162,897.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | MS110, monoclonal |
形式 | liquid |
包含 | ≤0.1% sodium azide as preservative |
species reactivity | human |
manufacturer/tradename | Calbiochem® |
储存条件 | do not freeze |
同位素/亚型 | IgG1 |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |