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Sphingosine kinase 2 (long form) Active human

品牌
Sigma-Aldrich
货号
SRP0287
规格纯度
recombinant, expressed in baculovirus, ≥80% (SDS-PAGE)
参考价格
8727.46 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Glucose activates sphingosine kinase 2 in pancreatic β cells leading to a rise in S1P levels, which is important for glucose-stimulated insulin secretion. Human sphingosine kinase 2 (GenBank Accession No.NM_020126), is full length with an N-terminal His tag, has a molecular weight of 74 kDa, and is expressed in a Baculovirus infected Sf9 cell expression system.

应用

Sphingosine kinase 2 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 2 has been used in a study to assess neuroapoptosis in the developing mouse brain. Sphingosine kinase 2 assays were used to confirm FTY720 bioactivity in a study investigating cranial allograft incorporation.

单位定义

One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRESNA.54

产品性质

重组expressed in baculovirus
测定≥80% (SDS-PAGE)
形式aqueous solution
specific activity≥20 units/μg
分子量 74 kDa
NCBI登记号NM_020126
运输dry ice
储存温度−70℃
Gene Informationhuman ... SPHK2(56848)

Sigma-Aldrich

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