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PRMT5/MEP50 Active human

品牌
Sigma-Aldrich
货号
SRP0146
规格纯度
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
参考价格
8831.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Protein arginine methyltransferase 5 (PRMT5) also referred to as Janus kinase-binding protein 1, is encoded by the gene mapped to human chromosome 14q11.2. PRMT5 enzyme, containing 637 amino acids, belongs to type II enzymes, which is a subclass of PRMT family. PRMT5 is predominantly expressed at distinct level in various organs including heart, muscle and testis.
Human PRMT5 (GenBank Accession No. NM_006109), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 73kDa, expressed in a Baculovirus infected Sf9 cell expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化/生理作用

Protein arginine methyltransferase 5 (PRMT5) catalyzes the symmetric dimethylation of arginine residues. It is implicated in various biological functions such as ribosome biogenesis, assembly of the Golgi apparatus, cellular differentiation, cellular proliferation, apoptosis, and germ cell specification. Irregular expression of PRMT5 leads to several type of cancers such as leukemia and lymphoma, gastric cancer, breast cancer and colorectal cancer. PRMT5 plays an important role in the regulation of cell growth, proliferation and apoptosis in epithelial ovarian cancer cell lines A2780 and SKOV3. PRMT5 enables nuclear factor-κB (NF-κB) activation via demethylating arginine 30 (R30) on the p65 subunit of NF-κB. PRMT5 participates in transcriptional regulation by methylating histones H2A, H3R8, and H4R3 as well as by inhibiting the expression of suppressor of tumorigenicity 7 (ST7), and nonmetastatic 23 (NM23) genes. PRMT5 enhances DNA damage response via methylation of tumor suppressor p53 on R333, R335, and R337 in the oligomerization domain.

单位定义

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37℃.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRESNA.32

产品性质

生物来源human
重组expressed in baculovirus infected insect cells
测定≥70% (SDS-PAGE)
形式aqueous solution
分子量 73 kDa
包装pkg of 20 μg
储存条件avoid repeated freeze/thaw cycles
浓度>0.02 mg/mL
NCBI登记号NM_006109
NM_024102
UniProt登记号O14744
运输dry ice
储存温度−70℃
Gene Informationhuman ... PRMT5(10419)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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