产品说明
应用
Useful for Phosphatase assay and Western blot.
生化/生理作用
Recombinant full length human PP2Aalpha and human PPP2R1A were expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).
重悬
Formulated in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
产品性质
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | 70-95% (SDS-PAGE) |
| 形式 | frozen liquid |
| specific activity | 3400-4600 nmol/min·mg (BIOMOL Green) |
| 分子量 | ~38 kDa ~65 kDa |
| 浓度 | 0.1 μg/μL |
| 登记号 | NM_002715 NM_014225 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PP2A(5524), PPP2R1A(5518) |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H319 - H360D |
| 预防措施声明 | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| 危险分类 | Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
