产品说明
应用
Useful for kinase assay and Western blot.
生化/生理作用
Full-length recombinant human PCTK3 (CDK18) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK3 or PCTAIRE protein kinase 3 is a member of the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK3 is thought to play a role in terminally differentiated neurons and it contains an N-terminal extension relative to CDC2 (1). PCTK3 is expressed in a variety of human cell lines and tissues (2). Elevation in the protein levels of PCTAIRE 3 in the temporal cortex of Alzheimer′s disease relative to control brains, have been observed. It is proposed that PCTAIRE 3 is a paired helical filaments-associated kinase that modulates tau phosphorylation.
重悬
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
产品性质
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | 70-95% (SDS-PAGE) |
| 形式 | frozen liquid |
| specific activity | 35-55 nmol/min·mg (radiometric) |
| 分子量 | ~64 kDa (Cyclin Y) ~76 kDa (PCTK3) |
| 浓度 | 0.1 μg/μL |
| 登记号 | BC011526 NM_145012 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... CCNY(219771), CDK18(5129) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
