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PDE8A1 Active human

品牌
Sigma-Aldrich
货号
SRP0273
规格纯度
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
参考价格
8831.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

PDE8A (phosphodiesterase 8A) is one of the two subfamilies of PDE8 protein family, the other being PDE8B. PDE8 shows wide range of tissue expression, and is especially abundant in testis. PDE8A contains a Per, ARNT and Sim (PAS) signaling domain in its N-terminal. PDE8s contain receiver (REC) domain, N-glycosylation, N-myristoylation, amidation consensus sites, and potential kinase substrate sites for PKC (protein kinase), PKA and casein kinase.
Human PDE8A1 (GenBank Accession No. NM_002605), full length with N-terminal GST tag, MW = 119kDa, expressed in a Baculovirus infected Sf9 cell expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化/生理作用

PDE8 (phosphodiesterase 8) are cAMP-specific enzymes, and they play a role in various processes such as in T-cell activation regulation, chemotaxis of activated lymphocytes, control of testosterone synthesis in Leydig cells, initiation of glucose-induced biphasic insulin response, steroidogenesis, and excitation–contraction coupling. rs2304418 variant in this gene is linked with lower mRNA expression of PDE8A, which in turn is linked with reduced HIV-1 (human immunodeficiency virus) replication. The expression of this protein is significantly high in macrophages, and is controlled by miR-145-5p. PDE8A promotes HIV-1 replication in macrophages and this is controlled at the level of reverse transcription.

单位定义

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37℃

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRESNA.32

产品性质

生物来源human
重组expressed in baculovirus infected insect cells
测定≥70% (SDS-PAGE)
形式aqueous solution
specific activity≥437 pmol/min-μg
分子量 119 kDa
包装pkg of 10 μg
浓度>0.02 mg/mL
NCBI登记号NM_002605
UniProt登记号O60658
运输dry ice
储存温度−70℃
Gene Informationhuman ... PDE8A(5151)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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