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L-Lactate Assay Kit

品牌
Sigma-Aldrich
货号
MAK443
包装型号
规格纯度
Sufficient for 100 fluorometric tests
参考价格
4876.23 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.

特点和优势

  • Detection limit of 1 μM and linearity up to 50 μM L-lactate in 96-well plate assay.
  • Convenient room temperature assay. The procedure involves adding a single working reagent, and reading the fluorescence after 60 minutes.
  • Compatible with high-throughput handling systems.

适用性

The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.

原理

The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.

产品性质

质量水平100
detection methodfluorometric
相关疾病diabetes; pulmonary disorders; cardiovascular diseases; cancer; endocrinological disorders
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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