产品说明
应用
Useful for kinase assay and Western blot.
生化/生理作用
Recombinant full-length human ERK3 (MAPK6) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. ERK3 (MAPK6) or mitogen-activated protein kinase 6 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases) which also known as extracellular signal-regulated kinases (ERKs) that are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals and tyrosine phosphorylated in response to insulin and NGF (1). ERK3 is highly expressed in various human tissues, most abundantly in skeletal muscle (2).
重悬
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
储存及稳定性
Store in aliquots at -70℃. Avoid freeze/thaw cycles.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
产品性质
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | 70-95% (SDS-PAGE) |
| 形式 | frozen liquid |
| specific activity | 20-30 nmol/min·mg (radiometric) |
| 分子量 | ~135 kDa |
| 浓度 | 0.05 μg/μL |
| 登记号 | NM_002748 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... ERK3(5597) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
