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DNMT3B/DNMT3L Active human

品牌
Sigma-Aldrich
货号
SRP0166
规格纯度
recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)
参考价格
8177.79 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) has two subfamilies-DNMT3B and ΔDNMT3B. These are expressed in many transcript variants. Several single-nucleotide polymorphisms are observed within the promoter region of the DNMT3B gene. The gene encoding this protein is localized on human chromosome 20q11.2. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is part of the DNA methyltransferases family. It has a cysteine-rich region containing a novel-type zinc finger domain. The gene encoding it is localized on human chromosome 21q22.3.

应用

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

生化/生理作用

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) is a de novo methyltransferase which targets the un-methylated 5′-C-phosphate-G-3′ (CpG) sites on the gene promoter. It is responsible for crucial epigenetic mechanisms for the regulation of chromosomal stability and for building a new methylation pattern. The protein is associated with promoter hypermethylation in tumorigenesis and lung cancer. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a non-catalytic accessory factor which is mainly responsible for the stimulation of DNA methylation. It helps the DNA methylation machinery to be positioned on properly chromatinized DNA regions. The protein also helps to provide uniform methylation patterns. It has an important role in spermatogenesis and mutations in the DNMT3L gene might be associated with azoospermia susceptibility.

外形

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 20% glycerol, 4 mM glutathione.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

NACRESNA.32

产品性质

生物来源human
重组expressed in baculovirus infected insect cells
测定≥90% (SDS-PAGE)
形式aqueous solution
分子量 27 kDa (DNMT3L)
59 kDa (DNMT3B)
包装pkg of 10 μg
浓度>0.02 mg/mL
NCBI登记号NM_006892 (DNMT3B)
NM_013369 (DNMT3L)
UniProt登记号Q9UBC3
运输dry ice
储存温度−70℃
Gene Informationhuman ... DNMT3B(1789)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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