产品说明
一般描述
CK1gamma3 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1gamma3 is a ubiquitously expressed protein kinase found in the nuclei, cytoplasm and membrane fractions of eukaryotic cells. CK1gamma3 preferentially phosphorylates acidic substrates using ATP as a phosphate donor. The kinase domain of CKI isoforms have been shown to associate with protein kinase C-potentiated inhibitor protein of 17kDa, called CPI-17. CPI-17 specifically inhibits myosin light chain phosphatase and this effect is potentiated when it is phosphorylated on Thr-38 by protein kinases such as the CK1 isoforms.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
基本信息
NACRES | NA.32 |
产品性质
重组 | expressed in baculovirus infected Sf9 cells |
产品线 | PRECISIO® Kinase |
测定 | ≥70% (SDS-PAGE) |
形式 | buffered aqueous glycerol solution |
specific activity | 17-23 nmol/min·mg |
分子量 | ~73 kDa |
NCBI登记号 | NM_001031812 |
运输 | dry ice |
储存温度 | −70℃ |
Gene Information | human ... CSNK1G3(1456) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |