产品说明
一般描述
Serine/threonine-protein kinase PLK4 (PLK-4) is a member of the protein kinase superfamily and plays an essential role in centriole duplication. PLK-4 triggers procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins. It may be involved in tumorigenesis since centrosome aberrations are frequently observed in tumors. PLK-4 may also be involved in trophoblast differentiation by phosphorylating HAND1, leading to the disruption between HAND1 and MDFIC and then activating HAND1.
免疫原
Linear peptide corresponding to Human PLK-4.
应用
Research Sub Category
Apoptosis - Additional
Immunocytochemistry Analysis: A representative lot detected PLK-4 in U20S cell lysates transfected with control and Cep152 siRNA, but not U2OS cell lysates transfected with PLK4 siRNA. (Cizmecioglu, O. et al. (2010). J Cell Biol. 191(4):731-739).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PLK-4 in HeLa cell lysates cotransfected with Flag-tagged Gcp6 and Myc-tagged Plk-4 (Bahtz, R. et al. (2012). J Cell Sci. 125 (Pt 2): 486-496).
Detect Polo Like Kinase (PLK) using this mouse monoclonal antibody, Anti-PLK-4 Antibody, clone 6H5 validated for use in western blotting, ICC & IP.
Research Category
Apoptosis & Cancer
质量
Evaluated by Western Blotting in U2Os cell lysate.
Western Blotting Analysis: 4.0 µg/mL of this antibody detected PLK-4 in 10 µg of U2Os cell lysate.
目标描述
109 kDa observed
外形
Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
U2Os cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 6H5, monoclonal |
| species reactivity | human |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG3κ |
| NCBI登记号 | NP_001177728 |
| UniProt登记号 | O00444 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
