产品说明
一般描述
The melanocyte-specific protein PMEL (also known as Pmel17, gp100, melanocyte protein, premelanosome protein or silver locus protein homolog (SILV) is a type I transmembrane glycoprotein that is expressed primarily in pigment cells of the eye and skin. In melanosomes, PMEL protein forms a fibrillar matrix on which the UV-shielding pigment melanin is deposited.1-2
特异性
Monoclonal Anti-PMEL antibody specifically recognizes human PMEL protein.
应用
The antibody may be used in various immunochemical techniques including Immunoblotting (~100 kDa), Immunohistochemistry and Immunofluorescence.
生化/生理作用
PMEL fibrils are a major functional component of the melanosomal compartment as they optimize the melanin polymerization, condensation and storage.1,3
In addition, PMEL fibrils have an amyloidogenic nature sharing features with pathological amyloids.4 Thus, PMEL is suggested as an excellent model system to study mechanisms of intracellular amyloid formation.1
Mutations in PMEL are associated with pigmentation disorders and/or impairments in eye development in various species.1,5,6
外形
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
储存及稳定性
For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 质量水平 | 200 |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | PL-19 |
| 形式 | liquid |
| species reactivity | human |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: 1-2 μg/mL using human melanoma SK-MEL-28 cell lysate immunofluorescence: 1-2 μg/mL using human melanoma SK-MEL-28 cells |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P40967 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
