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Anti-DLK1 Antibody, clone 3A10

品牌
Sigma-Aldrich
货号
MABN671
规格纯度
ascites fluid, clone 3A10, from mouse
参考价格
6190.47 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Protein delta homolog 1or DLK-1 (DLK1), also known as pG2 and the Preadipocyte factor-1 (Pref-1), is a single pass type 1 membrane protein known to play a steering role in neuroendocrine differentiation. The protein is expressed within the stromal cells in contact with the vascular structure of the placental villi, yok sac, fetal liver, adrenal cortex and pancreas during development and DLK1 is also expressed within the beta cells of the islets of Langerhans in the adult. DLK1 plays an important gene regulatory expression role during the development of not only these very early structures but also in the regulation of the development of many specialized cells and tissues such as adipocytes. In adipose tissue, DLK1/Pref-1 is specifically expressed in preadipocytes but not in adipocytes and thus can be used as a preadipocyte marker. The inhibition operates through the creation of a soluble cleaved cytoplasmic form of DLK1 via the action of the metalloproteinase ADAM17 on mature DLK1. Fetal Antigen 1 (FA1) or soluble DLK-1/Pref-1 activates the MAPK kinase/ERK pathway, and via Sox9 interactions ultimately inhibits the differentiation of pre-adipocytes into mature adipocytes. Furthermore, by inducing Sox9, Pref-1 promotes chondrogenic induction of mesenchymal cells but prevents chondrocyte maturation as well as osteoblast differentiation. Thus, DLK1/Pref-1 directs multipotent mesenchymal cells (MSCs) toward the chondrogenic lineage but inhibits differentiation into adipocytes as well as osteoblasts and chondrocytes. Naturally, DLK1 has multiple isoforms each with slightly differing but related influences on MSCs development. Finally, DLK1 is also expressed in various tumors including small cell lung carcinoma and numerous neuroendocrine cancers.

免疫原

Purified recombinant fragment of human DLK1 expressed in E. Coli.

应用

Detect DLK1 using this mouse monoclonal antibody, Anti-DLK1 Antibody, clone 3A10 validated for use in IHC & Immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected DLK1 in U251 cells.

Optimal working dilutions must be determined by end user.

质量

Evaluated by Immunohistochemistry Analysis in human pancreas tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected DLK1 in human pancreas tissue.

目标描述

45 calculated

外形

Unpurified

分析说明

Control
Human pancreas tissue

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆3A10, monoclonal
species reactivityhuman, mouse
technique(s)immunofluorescence: suitable
immunohistochemistry: suitable
同位素/亚型IgG1
UniProt登记号P80370
运输wet ice
Gene Informationhuman ... DLK1(8788)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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