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Anti-DNA Antibody, single stranded, clone 16-19

品牌
Sigma-Aldrich
货号
MAB3034
规格纯度
clone 16-19, Chemicon®, from mouse
参考价格
5520.38 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

Single stranded DNA (ssDNA) (poly-deoxythymidine). Shows 90% reactivity to ssDNA and 12% reactivity to dsDNA.

ANTIGEN BINDING PROFILE:

Relative Binding To:

Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53

a) Native calf thymus DNA
b) Heat-denatured calf thymus DNA

Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).

BINDING ASSAY

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.

ISOELECTRIC POINT = 8.3

免疫原

Epitope: single stranded DNA

应用

Research Category
Apoptosis & Cancer
ELISA
This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus.
Optimal working dilutions must be determined by end user.
Research Sub Category
Apoptosis - Additional

Cell Cycle, DNA Replication & Repair
Anti-DNA Antibody, single stranded, clone 16-19 is an antibody against DNA for use in ELISA.

外形

0.1 M Tris-Glycine (pH 7.4), 150 nM NaCl with 0.05% sodium azide.
Format: Purified

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆16-19, monoclonal
species reactivity (predicted by homology)all
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
同位素/亚型IgG2aκ
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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