Anti-CHIP Rabbit pAb

产品说明

一般描述

This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP.
Recognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively.
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein.

免疫原

a recombinant protein consisting of full-length human CHIP fused to a His•Tag sequence

包装

100 μL in Plastic ampoule

应用

Immunoblotting (1:200-1:1000)

Immunofluorescence (1:250)

Immunoprecipitation (1:20)

警告

Toxicity: Standard Handling (A)

外形

Undiluted serum.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
COS-7 cells

其他说明

May also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.
Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA99, 12847.
Demand, J., et al. 2001. Curr. Biol.11, 1569.
Connell, P., et al. 2001. Nat. Cell Biol.3, 93.
Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938.
Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.
Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

安全信息