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Alanine Racemase from Bacillus stearothermophilus

品牌
Sigma-Aldrich
CAS
9024-06-0
货号
SAE0023
规格纯度
buffered aqueous solution, ≥10 IU/mg solid
参考价格
5206.02 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

应用

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine †.

生化/生理作用

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†

单位定义

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 ℃ in a coupled assay system with L-alanine dehydrogenase.

外形

Aqueous solution containing 10 mM potassium phosphate

基本信息

酶学委员会(EC)编号5.1.1.1 ( BRENDA | IUBMB )
MDL编号MFCD03454300
NACRESNA.54

产品性质

质量水平200
生物来源Bacillus sp. (B. stearothermophilus)
形式buffered aqueous solution
specific activity≥10 IU/mg solid
分子量Mr 78 kDa (2 subunits 39 kDa each)
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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