产品说明
一般描述
Amicon® Ultra-2 mL centrifugal devices with Ultracel® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at 100% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.
应用
- Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates.
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
特点和优势
Minimum Final Concentrate Volume: 15 µL–70 µL
联系
Replaces: 4202
外形
Color Code: 透明
法律信息
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
eCl@ss | 36100101 |
NACRES | NB.24 |
产品性质
质量水平 | 400 |
物料 | polypropylene tube (for collection) regenerated cellulose membrane styrene-butadiene housing |
无菌性 | non-sterile |
产品线 | Amicon® |
manufacturer/tradename | Amicon® Ultra |
technique(s) | protein extraction: suitable protein purification: suitable |
长度 | 119.7 mm |
直径 | 15.9 mm |
过滤面积 | 1 cm2 |
孔径 | 3 kDa NMWCO |
试验参数 | sample volume: 2 mL |
运输 | ambient |