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A549 CELLS EGFR BIOSENSOR

品牌
Sigma-Aldrich
货号
CLL1097
包装型号
规格纯度
参考价格
50720.1 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

This cell line was derived from ATCC catalog No. CCL-185.

应用

The EGFR Biosensor cell line consists of the SH2 domain of the adaptor protein Grb2 fused to GFP. The biosensor was transduced into the A549 cell line and a stable cell line was cloned from a single cell. Within minutes after stimulation of the cell line with EGF, the biosensor shows robust redistribution towards the cell membrane and subsequent internalization through endocytosis. The internalization kinetics of the sensor-receptor complex can be quantified by image analysis software to count granules. The reporter response is highly selective as it can be induced only by specific EGFR ligands and is abolished by a selective inhibitor of EGFR, Tyrphostin AG 1478.
To learn more, please visit the EGFR Biosensor Cell Line webpage

特点和优势

Lentiviral transduction of DNA construct for stable expression of GFP-tagged SH2 binding domain.
These A549 cells are adherent with a doubling time of approx. 22 hours.

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

制备说明

Cell freezing medium-DMSO 1X, Cat. No. C6164
The base medium for this cell line is RPMI-1640, Cat. No. R0883. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F2442, to a final concentration (v/v) of 10%, L-glutamine, Cat. No. G7513, at a final concentration of 2 mM and puromycin, Cat. No. P9620, at a final concentration of 1 μg/mL.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃.
Media renewal changes two to three times per week.

基本信息

NACRESNA.81

产品性质

生物来源human male lung (Source Disease: Lung Carcinoma)
OMIM登记号131550
储存温度−196℃
Gene Informationhuman ... EGFR(1956), ERBB1(1956)

安全信息

储存分类代码6.2 - Infectious substances
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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