Acetylcholine Assay Kit

产品说明

一般描述

Acetylcholine is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood.

特点和优势

• 20 μL sample size.
• Linear detection range: colorimetric assay 10 to 200 μM, fluorimetric assay 0.4 to 10 μM acetylcholine.

适用性

The kit is suitable for the quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism in serum, plasma, urine, saliva, milk, tissue, and cell culture etc.

原理

The Acetylcholine Assay Kit provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase tobetaine and H₂O₂. The resulting H₂O₂ reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity at λ_Ex = 530 nm/λ_Em = 585 nm is directly proportional to the acetylcholine concentration in the sample.

产品性质

安全信息