产品说明
一般描述
The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.
特异性
Recognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.
免疫原
N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).
Epitope: N-terminus
应用
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western blot: 4 μg/mL
Immunohistochemistry (Acetone Fixed, Frozen)
ELISA
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.
联系
Replaces: AB1268
外形
Format: Purified
Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.
储存及稳定性
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
分析说明
Control
POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | Ali 12.28, monoclonal |
| species reactivity | human |
| should not react with | mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NM_000038.3 |
| UniProt登记号 | P25054 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
