产品说明
一般描述
Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.
特异性
This antibody recognizes BAK.
免疫原
KLH-conjugated linear peptide corresponding to human BAK at and around the N-terminus.
应用
Detect Bak (N-terminus) using this Anti-Bak (N-terminus) Antibody validated for use in WB, IH, IC & IP.
Western Blot (SNAP ID) Analysis: 1 - 4 µg/ml from a previous lot detected Bak on 10 µg of Hek293 cell lysate.
Immunoprecipitation Analysis: For unpurified antibodies. 10 µg from a previous lot immunoprecipitated Bak from 500 µg of NIH3T3 lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Bak in kidney tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Bak in HeLa, NIH/3T3, and A431 cells.
质量
Evaluated by Western Blot in Hek293 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Bak on 10 µg of Hek293 cell lysate.
目标描述
~ 24 kDa
联系
Replaces: 04-433
外形
Affinity purified
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | mouse, rat, human |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| NCBI登记号 | NP_001179 |
| UniProt登记号 | Q16611 |
| 运输 | wet ice |
| Gene Information | human ... BAK1(578) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
