产品说明
特异性
Recognizes MAP-2 (microtubule associated protein-2). MAP-2 is one of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (1).
MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (2,3). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (4,5).
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).
免疫原
Bovine brain microtubule protein.
应用
This Anti-MAP2 Antibody, Alexa Fluor 488 conjugated is validated for use in IH for the detection of MAP2.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Immunohistochemistry
Optimal working dilutions must be determined by end user.
APPLICATION NOTES:
Immunohistochemistry: Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
外形
Protein A purified
Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.
储存及稳定性
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Brain, Neuronal culture, Human glioblastoma T98G cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | ALEXA FLUOR® 488 |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | AP20, monoclonal |
| species reactivity | mouse, rat, bovine, chicken, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunohistochemistry: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NM_001039538.1 NM_002374.3 NM_031845.2 NM_031847.2 |
| UniProt登记号 | P11137 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
