产品说明
一般描述
Monoclonal Anti-Ral-A (mouse IgG1 isotype) is derived from the hybridoma RalA5F9.2 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. Ras-related protein Ral-A is a member of the Ras-like guanosine-5′-triphosphate (GTP) binding protein family. Ral-A protein is expressed in the islets of the pancreas, insulin-secreting cell lines and tissues during embryogenesis. The RALA gene is located on the human chromosome at 7p14.1.
特异性
Monoclonal Anti-Ral-A recognizes human, monkey, rat and mouse Ral-A, ~25 kDa.
免疫原
synthetic peptide corresponding to amino-acids 189-203 of human Ral-A, conjugated to KLH.
应用
Monoclonal Anti-RAL-A antibody produced in mouse may be used in:
- immunoblotting
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
生化/生理作用
Ras-related protein Ral-A can bind guanosine triphosphate (GTP) or guanosine diphosphate (GDP). It is known to possess a low intrinsic GTPase activity. Ral-A protein is involved in regulating the insulin secretion of pancreatic β-cells. This protein is also involved in membrane trafficking, oncogenic transformation and proliferation.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For extended storage, freeze at –20 ℃ in working aliquots. For continuous use, store at 2-8 ℃ for up to one month. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD08705597 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | RalA5F9.2, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 25 kDa |
| species reactivity | mouse, rat, human, monkey |
| 浓度 | ~2 mg/mL |
| technique(s) | immunocytochemistry: 5-10 μg/mL using HeLa cells indirect ELISA: suitable microarray: suitable western blot: 1-2 μg/mL using rat brain extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P11233 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... RALA(5898) mouse ... Rala(56044) rat ... Rala(81757) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
