产品说明
一般描述
STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases (1,2). In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT (1). Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1). After dimerization, the STATs accumulate in the nucleus by way of nuclear transport (1). STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production (1,2,3). The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.
特异性
Recognizes phosphorylated STAT5A/B atTyr694/Tyr699.
免疫原
KLH-conjugated linear peptide corresponding to phosphorylated human STAT5A/B at and around Tyr694/Tyr699.
Epitope: Tyr694/Tyr699
应用
Use Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 94-10C-9-10C-2 (rabbit monoclonal antibody) validated in WB to detect phospho-STAT5A/B (Tyr694/699) also known as signal transducer & activator of transcription 5A/B.
Research Sub Category
Transcription Factors
Transcription Factors
Research Category
Epigenetics & Nuclear Function
质量
Evaluated by Western Blot in U937 cell lysate.
Western Blot Analysis: 0.1 µg/ml of this antibody detected STAT5 A/B on 10 µg of U937 cell lysate.
目标描述
95 kDa
外形
Format: Purified
Protein A purified
Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 30% Glycerol.
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
分析说明
Control
GMCSF treated and untreated U937 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 94-10C-9-10C-2, monoclonal |
species reactivity | mouse, rat, human |
technique(s) | western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NP_003143.2 |
UniProt登记号 | P42229 |
运输 | dry ice |
Gene Information | human ... STAT5A(6776) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |