Anti-Vesicular Monoamine Transporter 2 Antibody

产品说明

特异性

Recognizes Vesicular Monoamine Transporter 2 (VMAT2). An antibody made to the C-terminal VMAT2 peptide detected a major band at ~55 kDa in postnuclear supernatants of CHO transfected with VMAT2 and not in wild type cells (Peter et al. 1995; Nirenberg et al. 1995). Some additional bands, both higher and lower molecular weight, were also detected and remain unaffected by the inclusion of protease inhibitors. Higher molecular weight bands may represent glycosylated VMAT2 (Peter et al. 1995; Nirenberg et al. 1995, Liu et al. 1994). VMAT2 has been localized in adrenal chromaffin cells, sympathetic ganglion cells, enterochromaffin-like cells of the stomach and in CNS (Peter et al. 1995; Nirenberg et al. 1995; Nirenberg et al. 1996). May recognize human due to the 92% homology between rat and human VMAT2.

免疫原

A 13 amino acid C-terminal peptide sequence from rat VMAT2 (Liu et al. 1992). This peptide is predicted to be cytoplasmic.

应用

Research Category
Neuroscience
Western blot: 1-10 μg/mL (Chemiluminescence technique)

Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 μg/mL (1 μg/mL VMAT1 peptide (Catalog Number AG251)/well)

Optimal working dilutions must be determined by end user.
Research Sub Category
Ion Channels & Transporters
Anti-Vesicular Monoamine Transporter 2 Antibody is an antibody against Vesicular Monoamine Transporter 2 for use in ELISA, IH & WB.

目标描述

55 kDa

外形

ImmunoAffinity Purified
Liquid in PBS with 0.1% BSA.

储存及稳定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Rat brain tissue

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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