产品说明
一般描述
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge.
特异性
Specific for rabbit IgG, heavy and light chain. The cross-reactivities of anti-rabbit IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.
应用
Goat Anti-Rabbit IgG Antibody, (H+L) HRP conjugate has been used in:
- immunohistochemistry (IHC)
- western blotting
- immunoprecipitation
生化/生理作用
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
质量
Western Blot, chromogenic:
1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).
Western Blot, chemiluminescent:
1:2,000-1:5,000 dilution can be used (Coligan, J., 1997).
外形
ImmunoAffinity Purified
Purified Goat IgG conjugated to horseradish peroxidase in buffer containing liquid in 0.01M PBS, pH 7.1 with 15mg/mL BSA and 0.1% Kathon.
储存及稳定性
The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.46 |
产品性质
| 质量水平 | 100 |
| 生物来源 | goat |
| 偶联物 | peroxidase conjugate |
| 抗体形式 | affinity purified immunoglobulin |
| antibody product type | secondary antibodies |
| 克隆 | polyclonal |
| species reactivity | rabbit |
| manufacturer/tradename | Chemicon® |
| 浓度 | 1 mg/mL |
| technique(s) | ELISA: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
| 同位素/亚型 | IgG |
| 运输 | wet ice |
安全信息
| 象形图 |   |
| 警示用语： | Warning |
| 危险声明 | H315 - H317 - H319 - H411 |
| 预防措施声明 | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 |
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
