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Anti-POU5F1 (Oct4) antibody produced in rabbit

品牌
Sigma-Aldrich
货号
P0056
规格纯度
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
参考价格
5045.46 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

POU class 5 homeobox 1 (POU5F1) recognizes defined octamer DNA element by a shared, conserved DNA-binding domain, namely the POU domain.
POU class 5 homeobox 1 (POU5F1), also known as octamer-binding transcription factor-4 (OCT-4), is expressed in embryonic stem cells and germ cells. It belongs to the POU family of transcription factors. Three splice variants of the protein (OCT4A, OCT4B and OCT4B1) have been identified. The gene encoding this transcription factor is localized on human chromosome 6p21.33.

免疫原

synthetic peptide corresponding to amino acids 19-34 of human POU5F1, conjugated to KLH. The corresponding sequence in rat is 81% homologous.

应用

Anti-POU5F1 (Oct4) antibody produced in rabbit has been used in Western Blotting.

生化/生理作用

POU5F1 or Oct4/Oct3 (Octamer-binding transcription factor), functions as an early developmental control gene. It has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and in primordial germ cells. It is down regulated in all differentiated somatic cell types both in vivo and in vitro. This is being accomplished through increased DNA methylation and structural changes involving the immediate upstream regulatory region. POU5F1 serves as a marker for germ cell tumors (GCTs) eliciting pluripotentiality, especially including embryonal, seminoma/ dysgerminoma/ germinoma.
POU class 5 homeobox 1 (POU5F1) may be a tumor initiating stem-like cell (TSC)-related marker. It has a role in stem cell pluripotency and self-renewal, and also modulates differentiation. The protein has been shown to be expressed in several cancers. POU5F1 is also vital for the induced pluripotent stem cell (iPSC) process.

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
分子量antigen ~43 kDa
species reactivityhuman
浓度~1.0 mg/mL
technique(s)immunoprecipitation (IP): 5-10 μg using NT2 cell lysate
indirect immunofluorescence: 2-5 μg/mL using paraformaldhyde-fixed F9 cells
western blot: 1-2 μg/mL using NT2 cell lysate
UniProt登记号Q01860
运输dry ice
储存温度−20℃
Gene Informationhuman ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)

安全信息

储存分类代码12 - Non Combustible Liquids
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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