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Anti-p53 antibody, Mouse monoclonal

Anti- p53 抗体,小鼠单克隆

品牌
Sigma-Aldrich
货号
P7874
规格纯度
clone DO-2, purified from hybridoma cell culture
参考价格
6523.48 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Monoclonal Anti-p53 (mouse IgG1 isotype) is derived from the hybridoma DO-2 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53.
p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

免疫原

recombinant human wild type p53. The antibody epitope resides between amino acids 10-16 of human p53.

应用

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for DNase I footprint, immunoprecipitation and western blotting.

生化/生理作用

p53 is phosphorylated at multiple sites by several protein kinases in response to DNA damage. It is essential for the maintenance of the non-tumorigenic phenotype of cells. Phosphorylation of p53 at Ser15 by ataxia telangiectasia mutate (ATM), ataxia telangiectasia and Rad3-related protein (ATR), and DNA-dependent protein kinase (DNA-PK) leads to a reduced interaction with its negative regulator MDM2 and accumulation of p53 protein. Checkpoint kinase 2 (Chk2) and Chk1 can phosphorylate p53 at Ser20, which enhances its activity, tetramerization, and stability. Elevation of p53 protein induces the transcriptional activation of multiple genes, including cyclin-dependent kinase inhibitor 1 (p21waf1).
Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆DO-2, monoclonal
形式buffered aqueous solution
分子量antigen ~53 kDa
species reactivityhuman
浓度~2 mg/mL
technique(s)immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using total cell extract of A431 cells
同位素/亚型IgG1
UniProt登记号P04637
运输dry ice
储存温度−20℃
Gene Informationhuman ... TP53(7157)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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