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Monoclonal Anti-PARP antibody produced in mouse

品牌
Sigma-Aldrich
货号
P248
规格纯度
clone C-2-10, ascites fluid
参考价格
11341 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Monoclonal Anti-PARP (mouse IgG1) is produced by immunizing mice with purified calf thymus poly (ADPribose) polymerase as the antigen. This antibody recognizes a 116kDa protein which corresponds to PARP and the 85kDa apoptosis induced cleavage product of prICE (proteinase resembling interleukin 1b-converting enzyme) and CPP32 (cysteine protease). It also recognizes PARP from mouse, rat, hamster and primate sources, but fails to detect avian PARP.
Poly (ADP-ribose) polymerase 1 (PARP1)/PARP is encoded by the gene mapped to human chromosome 1q42. It is a nuclear protein with molecular mass of 113kDa and is characterized with three functional domains. It belongs to the PARP superfamily.
PARP1 poly(ADP-ribose) polymerase 1) is also termed as ARTD1. It is an abundant nuclear protein that belongs to the PARP family. This gene is located on human chromosome 1q42.

特异性

The antibody reacts with PARP (116 kDa) and the 85 kDa apoptosis-induced cleavage product of prICE (proteinase resembling interleukin 1β-converting enzyme) and caspase-3 (CPP32 cysteine protease).

免疫原

calf thymus PARP. The epitope is found between amino acids 216-375 in the DNA-binding domain of PARP.

应用

Monoclonal Anti-PARP (Cleaved-Asp214) antibody has been used in western blotting and immunohistochemical staining.

生化/生理作用

PARP1 poly(ADP-ribose) polymerase 1) and its enzymatic product PAR controls cellular processes like DNA repair, chromatin remodeling, transcription and cell death. PARP1 regulates transcription. PARP1 can induce or block homologous recombination (HR).
Poly (ADP-ribose) polymerase 1 (PARP1)/PARP participates in DNA repair. It facilitates homologous recombination. PARP controls transcription by regulating chromatin structure, altering DNA methylation patterns, acting as a co-regulator of transcription factors and interacting with chromatin insulators. It also plays an important role in the protection of cardiovascular system. PARP1 is also implicated in the maintenance of genomic stability.

外形

Solution in mouse ascites fluid containing 0.02% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD01322527
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆C-2-10, monoclonal
分子量antigen 116 kDa (PARP)
antigen 85 kDa (prICE/caspase-3 cleavage product)
species reactivityprimate, mouse, hamster, rat
should not react with avian
technique(s)immunocytochemistry: 1:100-1:1,000
immunofluorescence: 1:100-1:1,000
immunohistochemistry: 1:10-1:100
western blot: 1:500-1:1,000
同位素/亚型IgG1
UniProt登记号P09874
运输dry ice
储存温度−20℃
Gene Informationmouse ... Parp1(11545), Parp2(11546)
rat ... Parp1(25591), Parp2(290027)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

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