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Anti-KAT2A (GCN5) Antibody, clone 2GC-2C11

品牌
Sigma-Aldrich
货号
MABE1791M
规格纯度
clone 2GC-2C11, from mouse
参考价格
1415.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Histone acetyltransferase KAT2A (UniProt: Q92830; also known as EC: 2.3.1.48, General control of amino acid synthesis protein 5-like 2, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97) is encoded by the KAT2A (also known as GCN5, GCN5L2, HGCN5) gene (Gene ID: 2648) in human. Histone acetyltransferase KAT2A belongs to the acetyltransferase family and is expressed in all tissues tested. However, its expression is highest in ovaries. It has three acetyl-CoA-binding regions (aa 579-581; 586-592; 617-620) and one bromodomain. It functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. It displays significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. KAT2A is also shown to acetylate non-histone proteins, such as CCAAT/enhancer-binding protein beta (CEBPB). In case of HIV-1 infection, histone acetyltransferase KAT2A is recruited by the viral protein Tat and it can regulates Tat′s transactivating activity and may help in inducing chromatin remodeling of proviral genes. Two isoforms of histone acetyltransferase KAT2A have been reported that are produced by alternative splicing. Isoform 2 lacks the first 410 amino acids.


特异性

Clone 2GC-2C11 detects Histone acetyltransferase KAT2A in human and murine cells. It targets an epitope within 15 amino acids from the N-terminal region.

免疫原

Ovalbumin-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Histone acetyltransferase KAT2A.

应用

Western Blotting Analysis: 2 µg/mL from a representative lot detected KAT2A (GCN5) in 10 µg of NIH/3T3 cell lysate.

Immunoprecipitation Analysis: A representative lot detected KAT2A (GCN5) in Immunoprecipitation applications (Brand, M., et. al. (2001). EMBO J. 20(12):3187-96).

Western Blotting Analysis: A representative lot detected KAT2A (GCN5) in Western Blotting applications (Riss, A., et. al. (2015). J Biol Chem. 290(48):28997-9009; Helmlinger, D., et. al. (2006). PLoS Biol. 4(3):e67; Brand, M., et. al. (2001). EMBO J. 20(12):3187-96).

Anti-KAT2A (GCN5), clone 2GC-2C11, Cat. No. MABE1791, is a highly specific mouse monoclonal antibody that targets Histone acetyltransferase KAT2A and has been tested in Western Blotting and Immunoprecipitation.

质量

Evaluated by Western Blotting in HeLa nuclear extracts.

Western Blotting Analysis: 2 µg/mL of this antibody detected KAT2A (GCN5) in 10 µg of HeLa cells nuclear extract.

目标描述

~94 kDa observed; 93.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2GC-2C11, monoclonal
species reactivityhuman, mouse
包装antibody small pack of 25 μg
technique(s)immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_066564
UniProt登记号Q92830

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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