产品说明
一般描述
CXCR3 is a 7-TM GPCR that is selective for the CXC chemokines IP10, ITAC and MIG (Loetscher et al., 1996). Binding of IP10 and MIG to CXCR3 induces Ca2+ mobilization, chemotaxis and inflammatory responses of T lymphocytes, and also act as potent inhibitors of angiogenesis. CXCR3 is highly expressed in IL-2-activated T lymphocytes in vitro (Loetscher et al., 1996), and in T lymphocytes present in inflamed tissues in rheumatoid arthritis and multiple sclerosis (Balashov et al., 1999; Qin et al., 1998). In vivo, neutralization of CXCR3 inhibits experimentally induced type I diabetes (Frigerio et al., 2002), peritonitis (Xie et al., 2003), and post-lung transplantation bronchiolitis obliterans syndrome (Belperio et al., 2002). Millipore’s ChemiScreenTM CXCR3 receptor membrane preparations are ideal tools for screening for antagonists of CXCR3 interactions with its ligands. With 0.1 nM 125I-labeled IP10, the CXCR3 membrane preparation at 5 μg/well gives a signal:bakground of greater than 10-fold.
TRANSFECTION: Full-length human CXCR3 cDNA
生化/生理作用
Target Sub-Family: Chemokine
GPCR Class: A
Protein Target: CXCR3
质量
Table 1. Signal:background and specific binding values obtained in a competition binding assay with CXCR3 membrane prep.
| 10 µg/well | |
|---|---|
| Signal:Background | 28.2 |
| Specific Binding (cpm) | 6028 |
SPECIFICATIONS: 1 unit = 5 µg
Bmax for [125I]IP-10 binding: 0.6 pmol/mg protein
Kd for [125I]IP-10 binding: ~ 0.2 nM
产品规格
Inucbation Conditions
RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] IP10 (Perkin Elmer #NEX348)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I-labeled IP10 at 0.1 nM
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32161000 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | human |
| 重组 | expressed in Chem-1 cells |
| manufacturer/tradename | ChemiScreen Chemicon® |
| 浓度 | 1.0 mg/mL |
| technique(s) | radioligand binding assay (RLBA): suitable |
| NCBI登记号 | NM_001504.1 |
| UniProt登记号 | P49682 |
| 运输 | dry ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
