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ChemiScreen Recombinant Human NMU1 Neuromedin Receptor

品牌
Sigma-Aldrich
货号
HTS062M
包装型号
规格纯度
参考价格
10463.8 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Neuromedin U (NmU) is a peptide characterized by its ability to promote smooth muscle contraction (Brighton et al., 2004a). Two GPCRs, NMU1 and NMU2, mediate the contractile effects of neuromedin U by activation of both Gq and Gi (Brighton et al., 2004b). In addition, NmU binds to NMU1 expressed on Th2 cells to induce cytokine release (Johnson et al., 2004). Chemicon′s NMU1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of NMU1 interactions with neuromedin U25. The membrane preparations exhibit a Kd of 0.08 nM for [125I]-neuromedin U25. With 5 μg/well NMU1 Membrane Prep and 0.5 nM [125I]-neuromedin U25, a greater than 30-fold signal-to-background ratio was obtained.
Human NMU1

应用

Radioligand binding assay, and GTPγS binding.
Human NMU1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

生化/生理作用

  • GPCR Class: A
  • Protein Target: NMU1
  • Target Sub-Family: Neuromedin U

产品规格

Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4℃.

Radioligand: [125I]-neuromedin U25 (Perkin Elmer # NEX383)

Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4℃.

外形

One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 30-fold signal:background with 125I-labeled neuromedin U25 at 0.5 nM.
Liquid in packaging buffer:

储存及稳定性

Maintain frozen at -70℃ for up to 2 years. Do not freeze and thaw.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

基本信息

eCl@ss32161000

产品性质

质量水平100
生物来源human
重组expressed in Chem-1 cells
manufacturer/tradenameChemiScreen
Chemicon®
technique(s)ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable
NCBI登记号NM_006056.3
UniProt登记号Q9HB89

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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