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ChemiSCREEN CCR1 Membrane Preparation

品牌
Sigma-Aldrich
货号
HTS005M
规格纯度
Human CCR1 GPCR membrane preparation for Radioligand binding Assays.
参考价格
10463.8 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Full-length human CCR1 cDNA
CCR1 is a GPCR that binds to a variety of CC ligands, including MIP-1a, RANTES, MCP-3, HCC-1, HCC-2, HCC-4, and MPIF-1 (Olson and Ley, 2002). Lymphocytes, macrophages, dendritic cells, and GM-CSF-activated neutrophils express CCR1 (Kaufmann et al., 2001; Cheng et al., 2001). Two selective, non-peptide small molecule antagonists of CCR1, BX-471 and CP-481,715, have been synthesized (Gladue et al., 2003; Liang et al., 2000). Pharmacological and genetic targeting of CCR1, either alone or in combination with cyclosporin A, reduces cardiac and renal allograft rejection (Gao et al., 2000; Horuk et al., 2001a; Horuk et al., 2001b), allergic encephalomyelitis (Liang et al., 2000), and renal fibrosis (Anders et al., 2002) in experimental models. Chemicon′s CCR1 Membrane Preparations are ideal for screening molecules that disrupt interaction between CCR1 and its ligands. The CCR1 Membrane Preps bind to MIP-1a with a Kd of 1.8 nM, and yield greater than 10-fold signal to background ratio with a MIP-1a concentration of 0.1 nM.

应用

Radioligand binding assay.

生化/生理作用

Protein Target: CCR1
Target Sub-Family: Chemokine
GPCR Class: A

质量

SPECIFICATIONS:
Bmax for [125I]MIP-1alpha binding: 3.3 pmol/mg protein
Kd for [125I] MIP-1alpha binding: ~ 1.8 nM HOST CELLS:

外形

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Each vial typically contains 500 units, where one unit is the amount of membrane prep that yields greater than 1000 cpm specific binding of 125I-labeled MIP-1α (0.1 nM input concentration).

储存及稳定性

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32161000
NACRESNA.84

产品性质

质量水平100
生物来源human
重组expressed in Chem-2 cells
manufacturer/tradenameChemiScreen
Chemicon®
浓度0.5 mg/mL
technique(s)radioligand binding assay (RLBA): suitable
NCBI登记号NM_001295.2
UniProt登记号P32246
运输dry ice
Gene Informationhuman ... CCR1(1230)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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