产品说明
一般描述
Monoclonal Anti-Myosin VI (mouse IgG1 isotype) is derived from the MUD-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to amino acids of human myosin VI. Myosin VI is a relatively abundant widespread unconventional myosin composed of an N-terminal motor domain, a light chain binding neck region, a coil-coiled region and a highly conserved C-terminal domain.
Myosins are actin-dependent motors that have 15 classes based on their phylogenetics . Myosin VI is an unconventional actin motor that moves towards the minus ends of actin filaments, which is opposite to the movement of conventional myosins.
特异性
Monoclonal Anti-Myosin VI antibody is specific for myosin VI in rats, humans and mice.
应用
Monoclonal Anti-Myosin VI antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoprecipitation
- western blot assay
- immunohistochemistry
生化/生理作用
Myosin VI is involved in the generation of cell shape change, cell motility, membrane remodeling and possibly in organelle and particle transport or tethering. It is also involved in membrane trafficking pathways in cultured mammalian cells where it is associated with the membrane ruffles and the trans-Golgi network. Myosin VI plays an essential role in the development of the mouse inner ear where it is expressed in the hair cell body, in the actin-rich cuticular plate at the base of the stereocilia and in the peri cuticular necklace region. Myosin VI gene mutations are involved in deafness and balance defects in the Snell′s Waltzer mouse. Myosin VI is present in the Golgi complex and may regulate vesicular transport across the membranes.
外形
Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02263784 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | MUD-19, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~150 kDa by SDS-PAGE |
| species reactivity | rat, human, mouse |
| 浓度 | ~1 mg/mL |
| technique(s) | immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 2 μg/mL using A431 total cell extract |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q9UM54 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... MYO6(4646) mouse ... Myo6(17920) rat ... Myo6(315840) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
