产品介绍：
产品说明
应用
3-甲基腺嘌呤(3-MA)用于抑制和研究多种条件下自噬(溶酶体自我降解)和细胞凋亡的机制。3-MA可通过抑制III型磷脂酰肌醇3-激酶(PI-3K)阻断自噬体的形成来对自噬进行抑制。 3-MA通常以5 mM的浓度用作自噬抑制剂。
在癌症生成过程中核酸甲基化检测的标准品。
3-Methyladenine has been used as an autophagy inhibitor:
- to determine the expressions of autophagy-related genes (Beclin 1, light chain 3 (LC3) and p62) using the transfected human bone marrow mesenchymal stem cells (BM-MSCs)
- with cisplatin to study the inhibition of autophagy influenced cisplatin-induced apoptosis in A2780cp cells
- to study the interplay between colistin-induced autophagy and apoptosis
包装
100, 500 mg in poly bottle
生化/生理作用
3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).
制备说明
该产品可溶于DMF(10 mg/mL；可能需要温和受热)。 受热情况下，其可以30 mg/ml的浓度溶于水、95％乙醇或1 N NaOH，但在冷却后会从溶液中析出。 可在DMSO中配制100 mM的3-MA溶液。
基本信息
| 经验(实验)分子式 | C6H7N5 |
| 分子量 | 149.15 |
| Beilstein | 146087 |
| EC 号 | 225-908-6 |
| MDL编号 | MFCD00010531 |
| PubChem化学物质编号 | 24897326 |
| NACRES | NA.51 |
产品性质
| 质量水平 | 300 |
| 测定 | ≥99% (HPLC) |
| 形式 | powder |
| mp | ~300 ℃ (dec.) (lit.) |
| 溶解性 | DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow |
| web metadata keyword.default | autophagy, autophagy inhibitor, autophagy assay, type III PI-3K inhibitor, PI-3K inhibitor, class III PI-3K inhibitor |
| SMILES string | Cn1cnc(N)c2ncnc12 |
| InChI | 1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3 |
| InChI key | FSASIHFSFGAIJM-UHFFFAOYSA-N |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
