产品说明
一般描述
Methionine aminopeptidase from Pyrococcus furiosus is a 32 kDa thermostable enzyme. It belongs to type 2a class of methionine aminopeptidase. Methionine aminopeptidase maintains protein homeostasis and coordinates posttranslational modification of proteins in eukaryotes.
X-ray crystallography of the structure of methionine aminopeptidase from Pyrococcus furiosus or PfMAP was performed at a resolution of 1.75A and showed that the protein consists of a catalytic domain containing two cobalt ions in the active site and a unique insertion domain which is specific to the prokaryotic form of the protein.
应用
Methionine Aminopeptidase from Pyrococcus furiosus has been used in a study to analyze the binding of Co(II)-specific inhibitors to the methionyl aminopeptidases from Escherichia coli and Pyrococcus furiosus. It has also been used in a study to examine the binding of a new class of pseudopeptide analog inhibitors.
生化/生理作用
Thermostable methionine aminopeptidase, which specifically liberates the N-terminal methioinine from proteins and peptides.
单位定义
One unit will hydrolyze 1 μmol of Met from Met-Pro-Ala-Ala-Gly in 1 minute at pH 7.5 at 37 ℃.
外形
Solution containing 0.01% Tween® 20, 0.1 mM CoCl2, and 10 mM Tris-HCl, pH 7.5.
法律信息
TWEEN is a registered trademark of Croda International PLC
基本信息
| 酶学委员会(EC)编号 | 3.4.11.18 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD03457470 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli |
| 测定 | ≥93% (SDS-PAGE) |
| 形式 | solution |
| specific activity | 0.5 units/mg protein |
| 分子量 | 37 kDa by SDS-PAGE |
| UniProt登记号 | P56218 |
| 异质活性 | Other proteases, none detected |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | Pyrococcus furiosus DSM 3638 ... PF0541(1468383) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
