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Methionine Aminopeptidase from Pyrococcus furiosus

品牌
Sigma-Aldrich
货号
M6435
规格纯度
≥93% (SDS-PAGE), recombinant, expressed in E. coli
参考价格
7334.1 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Methionine aminopeptidase from Pyrococcus furiosus is a 32 kDa thermostable enzyme. It belongs to type 2a class of methionine aminopeptidase. Methionine aminopeptidase maintains protein homeostasis and coordinates posttranslational modification of proteins in eukaryotes.
X-ray crystallography of the structure of methionine aminopeptidase from Pyrococcus furiosus or PfMAP was performed at a resolution of 1.75A and showed that the protein consists of a catalytic domain containing two cobalt ions in the active site and a unique insertion domain which is specific to the prokaryotic form of the protein.

应用

Methionine Aminopeptidase from Pyrococcus furiosus has been used in a study to analyze the binding of Co(II)-specific inhibitors to the methionyl aminopeptidases from Escherichia coli and Pyrococcus furiosus. It has also been used in a study to examine the binding of a new class of pseudopeptide analog inhibitors.

生化/生理作用

Thermostable methionine aminopeptidase, which specifically liberates the N-terminal methioinine from proteins and peptides.

单位定义

One unit will hydrolyze 1 μmol of Met from Met-Pro-Ala-Ala-Gly in 1 minute at pH 7.5 at 37 ℃.

外形

Solution containing 0.01% Tween® 20, 0.1 mM CoCl2, and 10 mM Tris-HCl, pH 7.5.

法律信息

TWEEN is a registered trademark of Croda International PLC

基本信息

酶学委员会(EC)编号3.4.11.18 ( BRENDA | IUBMB )
MDL编号MFCD03457470
NACRESNA.54

产品性质

质量水平200
重组expressed in E. coli
测定≥93% (SDS-PAGE)
形式solution
specific activity0.5 units/mg protein
分子量 37 kDa by SDS-PAGE
UniProt登记号P56218
异质活性Other proteases, none detected
运输dry ice
储存温度−20℃
Gene InformationPyrococcus furiosus DSM 3638 ... PF0541(1468383)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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