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Anti-GAL4, Activation domain antibody produced in rabbit

品牌
Sigma-Aldrich
货号
G9293
规格纯度
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
参考价格
5446.7 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Useful in the study of protein:protein interaction studies. GAL4 DNA activation domain fusion proteins.
GAL4 protein is an 881 amino acid transcription factor containing an independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a regulatory gene that belongs to GAL gene family. It is a component of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).

免疫原

Synthetic peptide corresponding to amino acids of Saccharomyces cerevisiae GAL4 protein.

应用

Anti-GAL4, Activation domain antibody produced in rabbit has been used in western blotting.

生化/生理作用

GAL4 induces the genes that are responsible for regulation of galactose metabolism in saccharomyces cerevisiae. GAL4 protein is widely used for studying transcriptional activation in eukaryotes. The yeast two hybrid system is widely used to study to discover protein− protein interactions (PPIs) and protein−DNA interactions.

外形

The product is provided as a solution of affinity isolated antibody in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD01633372
NACRESNA.56

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
浓度~1.0 mg/mL
technique(s)western blot: 0.5 μg/mL using GAL4-AD fusion protein in Saccharomyces cerevisiae extract
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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