产品说明
一般描述
β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.
应用
β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据,以优化不同来源的酶对不同药物类别的水解条件,并使用色谱纯化酶降低酯酶活性的影响,从而将6-MAM转化为吗啡
有效水解类固醇葡糖苷酸。
包装
25000, 100000 units in poly bottle
单位定义
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 ℃ at the pH 6.8 (30 min assay).
外形
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Highly purified solution in 50% glycerol
基本信息
酶学委员会(EC)编号 | 3.2.1.31 ( BRENDA | IUBMB ) |
EC 号 | 232-606-8 |
MDL编号 | MFCD00130629 |
NACRES | NA.54 |
产品性质
质量水平 | 200 |
重组 | expressed in E. coli |
形式 | aqueous glycerol solution |
specific activity | >20,000,000 units/g protein |
分子量 | 74 kDa |
浓度 | >1 mg/mL |
运输 | wet ice |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H317 - H334 |
预防措施声明 | P261 - P280 - P342 + P311 |
危险分类 | Resp. Sens. 1 - Skin Sens. 1 |
储存分类代码 | 13 - Non Combustible Solids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |